Nana

Nana is a minor antagonist of Madagascar and the secondary antagonist of Madagascar: Escape 2 Africa. She is the archenemy of Alex.

She is voiced by Elisa Gabrielli.

Madagascar
In the first movie, Nana is an elderly human female who encounters Alex the Lion in Grand Central Station, where she assaults him and angrily calls him a "bad kitty". She also hits Melman with her hand bag, causing his head to get stuck in the Grand Central Station Clock. When the animals are surrounded by police, she somehow gets past and kicks Alex in "the batteries" (or his groin). She was quickly taken away by police. As shown in the sequel, she was not arrested, as she was interviewed as a witness.

Madagascar: Escape 2 Africa
In Madagascar: Escape 2 Africa, Nana has a larger role, where she is the secondary antagonist. She becomes the leader of an army of tourists. She gets into another fight with Alex, and wins again, although Alex puts up much more of a fight this time. At the end of the film, Alex gives Makunga Nana's handbag. When Nana sees Makunga holding it, she starts kicking, stepping, hand-whacking, wet-willying, arm-burning, and spanking him before taking him away.

Trivia
one tough cookie, and she responds by saying: "Brownies, troop 416, Yonkers". There is really a girl-scout group called the Brownie Scouts in certain cities in America, but it was never explained if she's a former member of them, a den-mother, or just comparing herself to a literal cookie. (Possibly all three, as she has great camping skills and is perky despite getting everyone lost in the wild looking for shelter).
 * However another Nana is seen in Shrek called Old Woman.
 * In the movie "Escape 2 Africa", one of the tourist called her
 * she did not appear in Madagascar 3 possibly died of her old age