Daughters of Aku

The Daughters of Aku are seven human women who work as the assassins of Aku and are the antagonists of the fifth and final season of Samurai Jack. Until six of them were killed, leaving Ashi the last living sister.

Born and raised by their mother, the "High Priestess", they were trained to do one thing, and that is to kill Samurai Jack.

Appearance
Being septuplets, they all share very similar physical traits consisting of black hair, slanted eyes, and black latex suits. Their only distinguishing traits amongst each other is their varying hairstyles. When out on their mission, they wear white masks.

In Season Five
The seven daughters were birthed by The High Priestess of an all female cult of Aku. After birth, the priestess had them raised as assassins to succeed where many others have failed in the mission of killing Jack. They were put through the most brutal of training, designed to sculpt them into the ultimate warriors, such as facing a hulking, muscle bound opponent when they were mere children. Even more, they have been utterly taught to think that Aku is the true savior of the planet and Jack seeks to destroy all that Aku has "created". When their training was complete, they donned the masks and head coverings, and set out to fulfill their mission.

As of episode 4, six sisters have been confirmed deceased except for Ashi, Leaving her the sole survivor of the Daughters of Aku. Initially hostile to one another, Jack and Ashi are forced to set aside their enmities to escape from a gigantic creature that swallowed them whole. When they did escape Ashi attempted to attack Jack from behind. But Seeing cherishing a pair of bugs and sparing them their lives Ashi, remembering her own past memory (her mother, the High Priestess killed bugs in contrast to Jack), declined to kill him, hinting Ashi's possible defection in the future episodes.