I can think of so many

Handsome Jack from Borderlands

GLaDOS, Wheatly, and Cave Johnson from Portal

Fawful and Bowser from super mario

Hades from Kid Icarus Uprisng

The original three villains from the ratchet and clank series

I think Trevor Philips and Brucie Kibbitz from GTA

Dr. Eggman from Sonic

Dr. Wily from Megaman

King K Rool from DK

And King Dedede from Kirby

Trevor Philips is the best.

Bowser

Joseph Seed and the Seed family. Joseph in particular because there is something about religious fanaticism and corruption that is very powerful. Given that people have used religion to justify everything from murder to war. I only recently started playing the game but I know when I saw Joseph staring at me and reaching his hands out to be arrested I was genuinely terrified. It was the way he looks at you, his character design is amazing and his voice is calm, trusting even. There’s something about his character that causes you to become enraptured with him even though you also know that he can and will commit various atrocities and think it all in the plan and path to salvation. Not to mention he does in some ways, remind me of Hans Landa from ‘Inglorious Bastards’ and Hans has always been one of my favorite villains.

Vladimir makarov from original modern warfare series

Wilhelm deathshead strasse from wolfenstien the new order

Nemesis from resident evil 3

Wallace Breen from half life 2

Bendy from bendy and the ink machine

Mael radec from killzone 2

Gravemind from halo

Olivia pirece from doom 2016

And frank tenpenny from GTA San Andreas

General John Hemlock from Dead Rising 3. Dr. Nefarious Tropy and Dr. Nefarious Tropy(Female) from Crash Bandicoot 4: It’s About Time. Cesare Borgia from Assassin's Creed: Brotherhood. Pontiff Sulyvahn from Dark Souls III. Stefano Valentini from The evil within 2 Alduin from The Elder Scrollc V Skyrim

Dr. Malcom Betruger from Doom 3 Senator Steven Armstrong from Metal Gear Rising: Revengeance.

My favorite villain is Lionel starkweather from manhunt

Trevor Phillips is not a antagonist he is one of the 3 main protagonists just like Franklin and Michael. The real antagonist's are Steve Haines and Devin Weston.

Yeah but out of the three protagonists Trevor is probably the most evil.

Also Trevor isn’t on the hero’s wiki even though Franklin and Michael are