King Olly

12: Waluigi (Mario Tennis)

11: Wario (Super Mario Land 2: 6 Golden Coins)

10: Bowser Jr. (Super Mario Sunshine)

9: Kamek (Super Mario World 2: Yoshi's Island)

8: Bowser (Super Mario Bros.)

7: Count Bleck (Super Paper Mario)

6: King Olly (Paper Mario: The Origami King)

5: Scissors (Paper Mario: The Origami King) though he has no gender

4: King K. Rool (Donkey Kong Country) he's related to the Mario Games by the way

3: Antasma (Mario & Luigi: Dream Team)

2: King Boo (Luigi's Mansion)

1: Dimentio (Super Paper Mario)

SPOILERS ON THE 2ND ONE!

Mine is king Olly and Reclusa.

King Olly is a badass lookin paper one who has a younger sister named Olivia and he was the antagonist of paper mario: the origami king. At the end of the finale, he was dying and he tells his younger sister to fold his body as the 1000th and dies, I cried.

Reclusa was a plotwist villain from mario and Luigi brothership,(Same as dimento from super paper mario), he was the one who turn Cozette into a villain named Zokket, he was hatched from an egg like Pokémon, his name was based by that spider who is also named Reclusa, but at the end, he dies by saying his last words "But... But... I... I still..." and fades away (Just like the other heroes or villains from other fandoms), I feel so bad at Reclusa's death.