The Amoeba Boys are recurring antagonists in The Powerpuff Girls franchise.
They were voiced by the late Chuck McCann.
The Amoeba Boys are considerate to be the least malevolent major villains on the show, as they generally have a friendly relationship with the Powerpuff Girls on a few occasions. Even though they are generally harmless villains, but they do unintentionally cause problems to both the girls and the people of Townville, such in the episode "Geshundfight", when they foolishly stand all night on a grassy park with a sign "Keep Off the Grass" so they could get into trouble, but they fell asleep and easily caught a cold, which merges with their DNA, and created a virus that swirly spreads over Townsville and made everyone in the city severely ill. However, the Powerpuff Girls soon became infected with virus, and Professor Utonium required the girls' DNA to make a vaccine and soon cured everyone, including the girls and the Amoeba Boys. Despite what happened, the girls and the professor thank the Amoeba Boys for their cooperation while the Amoeba Boys were disappointed that they were not going to be punished for actions, since they caused the pandemic from the start.
Their greatest crime that they committed was in "Divide and Conquer" when they manage to steal an orange and split it into more pieces, they realized their ability to duplicate themselves and formed an army to successfully steal every orange in Townville and cause anyone to get infected with scurvy. However, they were defeated when the Powerpuff Girls merge all of their copies back together and the Amoeba Boys were finally sent to jail for their worst act of crime.
See Me, Feel Me, Gnomey
The Amoeba Boys appeared in the rock musical episode "See Me, Feel Me, Gnomey", where, like other villains, they are seen causing chaos and terrorizing people in Townsville. Yet, when it comes the Gnome, he is responsible for eliminating all the villains, including the Amoeba Boys, but later, when all the villains revive, the Gnome loses power.
They are also seen along with all the villains singing "Why Can't We All Get Along?".
The Powerpuff Girls Rule!
The Amoeba Boys appeared in "The Powerpuff Girls Rule!", trying to get the key to the world and take over Townsville. They were all seen attacking the other villains and Powerpuff Girls in attempt to get the key. During the race to get the key, The Amoeba Boys could not even get their own car so they just called a taxi. At the end of the episode, they all got beaten up.