Villains who regularly or only battle Spider-Man in any form of his media.
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A
- A.I.M. (Marvel)
- Aaron Davis (Earth-42)
- Aaron Davis (Marvel Cinematic Universe)
- Abomination (2010 Marvel Animated Universe)
- Abomination (Marvel)
- Absorbing Man (Marvel)
- Adolf Hitler (Marvel)
- Adrian Toomes (Ultimate Spider-Man game)
- Aftershock (Marvel)
- Agony (Marvel)
- Akasha Martinez
- Alchemax (Spider-Verse Films)
- Alistair Smythe (Marvel)
- Alistair Smythe (Spider-Man: The Animated Series)
- Alistair Smythe (The Amazing Spider-Man Video Games)
- Angelo Fortunato
- Annihilus (2010 Marvel Animated Universe)
- Apocalypse Gang
- Apocalypse Leader
- Arcade (Marvel)
- Armadillo (Spider-Verse Films)
- Arnim Zola (2010 Marvel Animated Universe)
- Arnim Zola (Marvel)
- Arsenic Candy Gang
- Ashley Kafka (The Amazing Spider-Man)
- Atrocity
- Attuma (2010 Marvel Animated Universe)
- Avatarr
- Awesome Android
B
- Banjo
- Baron Mordo (Marvel)
- Baron Mordo (Spider-Man: The Animated Series)
- Baron Zemo (Marvel Cinematic Universe)
- Baron Zemo II
- Bart Hamilton
- Basilisk (Marvel)
- Batroc the Leaper
- Bedlam
- Beetle (Marvel)
- Beetle (Ultimate Spider-Man game)
- Bella and Rita
- Ben Reilly (Marvel)
- Bestman Salvage
- Betsy Schneider (Marvel's Spider-Man)
- Beyonder (Marvel)
- Big Man (Marvel)
- Big Wheel (Spider-Man: The Animated Series)
- Black Cat (Marvel's Spider-Man)
- Black Cat (Marvel)
- Black Cat (Spider-Man: Web of Shadows)
- Black Cat (The Amazing Spider-Man Video Games)
- Black Cat (The Spectacular Spider-Man)
- Black Hole (Marvel)
- Black Mamba (Marvel)
- Blackheart (Marvel)
- Blackie Drago
- Blitz (Marvel)
- Blizzard (Marvel)
-
- Bolivar Trask (Marvel)
- Bolivar Trask (Ultimate Spider-Man game)
- Bonnie (Spider-Man and His Amazing Friends)
- Boomerang (Marvel Comics)
- Boomerang (Ultimate Spider-Man game)
- Bowman
- Brotherhood of Evil Mutants
- Brothers Grimm
- Bulldozer (Marvel)
- Bullseye (Marvel)
- Bullseye (MAX)
C
- Calypso (Marvel)
- Calypso (Raimiverse game adaptations)
- Carlton Drake (Marvel)
- Carlton Drake (Sony's Spider-Man Universe)
- Carnage (2010 Marvel Animated Universe)
- Carnage (Halloween Horror Nights)
- Carnage (LEGO Marvel Super Heroes)
- Carnage (Marvel)
- Carnage (Sony's Spider-Man Universe)
- Carnage (Spider-Man & Venom: Maximum Carnage)
- Carnage (Spider-Man 2000)
- Carnage (Spider-Man and Batman: Disordered Minds)
- Carnage (Spider-Man: Shattered Dimensions)
- Carnage (Spider-Man: The Animated Series)
- Carnage (The Amazing Spider-Man Video Games)
- Carnage (Ultimate Spider-Man game)
- Carnom
- Carrion (Marvel)
- Cash Register Thief
- Chameleon (Marvel's Spider-Man)
- Chameleon (Marvel)
- Chameleon (The Amazing Spider-Man Video Games)
- Chance (Marvel)
- Chitauri
-
-
- Cole Wittman (Marvel's Spider-Man)
- Colonel America
- Constrictor
- Crime Master (Spider-Man Noir)
- Crime-Master
- Crimson Dynamo
- Crossbones (Marvel)
D
- D.K. (Marvel)
- Daemos
- Daken
- Daredevil (Marvel Zombies)
- Darren Shipman
- Deadpool (2010 Marvel Animated Universe)
- Deadpool (Marvel)
- Deadpool (Spider-Man: Shattered Dimensions)
- Deadpool (Ultimate Marvel)
- Deadpool's Fan Club
- Death Adder (Marvel)
- Deathwatch
- Demogoblin (Marvel)
- Demogoblin (Spider-Man: Battle for New York)
- Dennis Carradine
- Diablo (Marvel)
- Dirty Cops
- Doctor Bong
- Doctor Doom (LEGO Marvel Super Heroes)
- Doctor Doom (Marvel Animated Universe)
- Doctor Doom (Marvel Zombies)
- Doctor Doom (Marvel)
- Doctor Faustus
- Doctor Octopus (2010 Marvel Animated Universe)
- Doctor Octopus (LEGO Marvel Super Heroes)
- Doctor Octopus (Marvel Noir)
- Doctor Octopus (Marvel's Spider-Man)
- Doctor Octopus (Marvel)
- Doctor Octopus (Raimiverse)
- Doctor Octopus (Spider-Man 2000)
- Doctor Octopus (Spider-Man 2017)
- Doctor Octopus (Spider-Man: The Animated Series)
- Doctor Octopus (Spider-Verse Films)
- Doctor Octopus (The Amazing Adventure of Spider-Man)
- Doctor Octopus (The Spectacular Spider-Man)
- Doctor Octopus' Tentacles (Raimiverse)
- Doctor Strange (Zombie)
- Donald Menken
- Donald Menken (The Amazing Spider-Man)
- Doom-Bots (Marvel)
- Doppelganger (Marvel)
- Dormammu (2010 Marvel Animated Universe)
- Dormammu (Marvel)
- Doug (Marvel Cinematic Universe)
- Dr. Mendel Stromm (Raimiverse game adaptations)
- Dr. Stillwell (Raimiverse game adaptations)
- Dracula (Marvel)
- Dragon Tail Leader
- Dreadknight
- Dreadpool
E
- Eddie Brock (Marvel)
- Eddie Brock (The Spectacular Spider-Man)
- Egghead (Marvel)
- Electro (2010 Marvel Animated Universe)
- Electro (Marvel's Spider-Man)
- Electro (Marvel)
- Electro (PlayStation Games)
- Electro (Raimiverse game adaptations)
- Electro (Spider-Man: The Animated Series)
- Electro (Spider-Man: The New Animated Series)
- Electro (Spider-Man: Web of Shadows)
- Electro (The Amazing Spider-Man)
- Electro (The Spectacular Spider-Man)
- Electro (Ultimate Spider-Man game)
- Elemental Fusion (Marvel Cinematic Universe)
- Elementals (Marvel Cinematic Universe)
- Enforcer (Marvel)
- Enforcers (Marvel)
- Enforcers (The Spectacular Spider-Man)
- Ezekiel Sims (Sony's Spider-Man Universe)
- Ezekiel Stane (Marvel)
F
G
- Gabriel Stacy
- Galactus (Lego Marvel Superheroes)
- Galactus (Marvel: Ultimate Alliance)
- Gentleman (Marvel)
- Ghost (Marvel)
- Giant-Man (Marvel Zombies)
- Goblin (Spider-Man: Shattered Dimensions)
- Godzilla (Marvel)
- Gog (Marvel)
- Goldbug (Marvel)
- Gorilla Man
- Grandmaster (Marvel)
- Grant Ward (Marvel)
- Graviton (Marvel)
- Great Gambonnos
- Green Goblin (2002 Novelization)
- Green Goblin (Earth-98311)
- Green Goblin (Green Goblin Mode)
- Green Goblin (Marvel Zombies)