2nd Dimension Charlene Doofenshmirtz is 2nd Dimension Doofenshmirtz's wife, mentioned only in Phineas and Ferb: Across the 2nd Dimension, and later as one of the two main antagonists (the other is her husband) in Tales From the Resistance: Back to the 2nd Dimension. She is an alternate reality version of Charlene Doofenshmirtz, the ex-wife of Heinz Doofenshmirtz and the mother of Vanessa Doofenshmirtz. Unlike her original counterpart (who is good-natured and unaware that Heinz is an evil scientist), 2nd Charlene is shown to be very evil and intelligent, sharing much of 2nd Doofenshmirtz's personality in general.
Across the 2nd Dimension
In the TV film, 2nd Charlene seems to be divorced with 2nd Heinz (who was the infamous Tri-State Area dictator in their dimension). She is only mentioned twice in the scene "Vanessa meets Doof", where 2nd Vanessa confronts her father for banishing her boyfriend Tony Marzulo for showing an interest in her. As 2nd Vanessa leaves for 2nd Charlene's penthouse by taking 2nd Doofenshmirtz's blimp to get there, 2nd Doofenshmirtz tells 2nd Vanessa to tell 2nd Charlene that she had fun though. This scene was cut from the film, though it was later shown in the movie's DVD through the bonus features.
Tales from the Resistance
2nd Charlene makes her first appearance in the episode sequel, which takes place two months following her ex-husband's arrest for his crimes against humanity. It has been reported that much of O.W.C.A.'s agents have gone missing during 2nd Doofenshmirtz's reign, and that they have captured the reformed Platyborg following an attack on Danville Park by two giant insect robots. Several of the members include 2nd Isabella's pet chihuahua Pinky (known as Chihuahuaborg) and Peter the Panda (known as Pandaborg). 2nd Dimension Candace Flynn (the leader of the Resistance) has been informed about the missing O.W.C.A. agents, so she paid a visit to 2nd Doofenshmirtz in jail, where he confessed that 2nd Charlene was the one responsible for kidnapping them and turning into cyborgs, similar to how he turned Perry into Platyborg. 2nd Candace then decides to track 2nd Charlene down and bring her to justice, even forcing 2nd Doofenshmirtz to help her by threatening to take his toy train away while he can still remain in prison.
Upon arriving at 2nd Charlene's penthouse with 2nd Phineas and Platyborg, 2nd Candace soon learns that 2nd Charlene is still married to 2nd Heinz and actually shares much of his evilness. It also turns out that both 2nd Doofenshmirtz and 2nd Charlene had pretended to be divorced because of several financial advantages (such as tax records, coupon mail, junk yard sales, and separate vacation trips) during 2nd Doofenshmirtz's reign. It is also revealed that that the park attack and Platyborg's kidnapping were nothing but ruses to trick Candace into freeing 2nd Doofenshmirtz and getting him back to her penthouse.
Taking the opportunity, 2nd Charlene had managed to capture 2nd Ferb and turned him into a cyborg, allowing her to capture 2nd Candace, 2nd Phineas, and Platyborg in her lair and freeing 2nd Doofenshmirtz. 2nd Charlene is also annoyed of having her husband of setting his focus on his silly childhood toy train so she throws it away, resulting 2nd Doofenshmirtz to go back to his evil ways.
However, it later turns out that 2nd Ferb had pretended to be a cyborg so that he can help the Resistance infiltrate into 2nd Charlene's penthouse with a frontal attack. As such, the Resistance arrive to free all of the animal agents with specialized fedora hats (equipped with magnetic deprogramming chips) while 2nd Candace engages into a bo staff fight with 2nd Charlene. After all of the agents are freed from their evil programming (except for Pandaborg), 2nd Candace is about to take both 2nd Doofenshmirtz and 2nd Charlene into custody. However, the arriving 2nd Vanessa tricks 2nd Candace into having a moment with her evil parents, which allowed the Doofenshmirtz family and Pandaborg to escape in a hover car driven by Tony, much to 2nd Candace's anger. As the notorious Doofenshmirtz family escape, 2nd Vanessa introduces Tony to both 2nd Charlene and 2nd Doofenshmirtz, the latter recognizing him as the boy he once banished earlier because of his interest towards 2nd Vanessa.