The Charlie Bugster emerged from the body of Kuroto Dan who had infected himself with the Bugster virus to evade the raid on his hideout by the Ministry of Health. After being fought off by Kamen Rider Ex-Aid, Charlie retreated inside Kuroto before emerging again to fight Ex-Aid, who quickly destroyed him with a Mighty Double Critical Strike. His debris was promptly collected by Kuroto in the Gashacon Bugvisor.
Charlie was later revived by Parado and used to complete Kamen Rider Chronicle, appearing as a boss within the game. One of the players to fight him was Teruyo Izumi, who became a Ride-Player to free her son who had been reduced to data by the game after receiving a game over. Charlie was later destroyed again by Ride-Player Nico.