The Cy-Bug Queen is a major antagonist in the Wreck-it-Ralph franchise. She appeared as the main antagonist and final boss of Wreck-it-Ralph: The Video Game. She is the supreme leader of the Cy-Bugs.
In the game, the Cy-Bugs began to hatch and invade Sugar Rush relentlessly, eventually heading to the Game Central Station. Vanellope and Calhoun evacuate with the other denizens while Ralph and Felix are given the task of destroying the bugs. Ralph and Felix journey off into Fix-It Felix Jr., Hero's Duty, and Sugar Rush to eradicate the bugs and eventually being led to The Game Central Station, where they must battle and fully defeat the Cy-Bug Queen through the use of massive grappling guns.
After the Queen is defeated, Ralph heads to the Sugar Rush game to celebrate the new holiday that was created by Vanellope, and then returning to his own game.
- Although King Candy crowns himself the leader of the Cy-Bugs after being eaten by one by accident, the Cy-Bug queen does not appear in the movie until the events of "Wreck-It Ralph: The Video Game" as the main villain. However, the events tied to the game could be considered non-canonical.