After saving Metro City, Megamind realizes that Tighten and the Doom Syndicate have ransacked his lab. Megamind decides to defeat Destruction Worker, Psycho Delic and Hot Flash at the Downtown, Midtown and Uptown sections of Metro City. Tighten took Metro Man and Megamind's DNA to become Blue Tighten. Megamind took care of Blue Tighten with his patented spray.
Mega Team Unite
Tighten redeems himself and helps Megamind, Minion and Metro Man take on the Doom Syndicate. This time, the Doom Syndicate has two extra members: Judge Sludge and the Conductor. The Mega Team defeats all five members of the Doom Syndicate and have saved Metro City.
- Tighten (Former Leader)
- Psycho Delic (Leader)
- Destruction Worker
- Hot Flash
- The Conductor
- Judge Sludge
- Lady Doppler
- The Doom Syndicate were originally going to appear in the original film but was cut for a streamlined plot.
- Concept art of the Doom Syndicate can be found in a Megamind art book.
They are parodies of DC Comics' Legion of Doom, Secret Society of Super Villains, Anti-Justice League, Brotherhood of Evil, Injustice League, Rogues, Terror Titans, Legion of Super-Villains, Injustice Gang, Marvel Comics' Masters of Evil, Sinister Six, Frightful Four, Brotherhood of Evil Mutants, Sinister Syndicate, Lethal Legion, Amalgam Comics' Sinister Society and Frightful Five, Lamos from totally spies, White Shadow Organization from minorteam, Meta-Bredd from static shock, Vicious Circle from savage dragon, Injustice Friends from dexter's laboratory, Mon*Star's Mob from silverhawks, Cyberdata from cyberforce, League of Villains from my hero academia, Arachnos from city of heroes/city of villains Bad-7 from disney's hero squad, Faculty 4 from codename: kids next door, C.R.O.O.K.S. from cops and Al-Ashrar from the 99.