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Colonel Matilda West is the main villainess from "Bad Science", episode 2.06 of Femme Fatales. She is the evil doppelgänger of the episode's main protagonist, scientist Matilda West.

She was played by Ashley Noel.

In the episode, Matilda was transported to an alternate dimension during her latest experiment regarding teleportation; an experiment performed with the help of her fiancee, Nick, her former flame (and Nick's brother), Aaron, and Aaron's girlfriend, Abby. The alternate world is ravaged by war and death, and it was there that Matilda met her doppelganger, Colonel Matilda West, the leader of the First Continental Alliance Army. The colonel accused Matilda of being a spy, but both of them ended up transported together with one touch.

With both of them back in the real world, Matilda decided to display Col. West as proof that her experiment works on living organisms. Colonel West took a look at Matilda's world and was amazed of how beautiful it looked compared to her world, all the while making a friendly impression on Aaron and Abby--both of whom disliked the real Matilda. She engaged in a romantic encounter with Aaron and later conversed with Abby, but shortly afterwards, both Matildas began to weaken, and it was Nick who concluded that their conditions were due to them being on the same world, meaning that Colonel West had to return to her own alternate reality. Before the process took place, Aaron spotted Abby attempting to switch the Matildas so the real one could go and the colonel could remain, and he switched them back.

In the episode's final scenes, however, Abby revealed to Aaron that while she was considering switching the Matildas, she didn't go through with it. This meant that the real Matilda went to the alternate world, while her evil doppelgänger remained, and Nick realized this after making love with who he thought was Matilda--when his lover stated that she didn't want to be in charge anymore. Knowing Matilda's true nature, Nick was shocked to realized that he was with Colonel West, and in the end of the episode, the evil Colonel West killed Nick, as well as Abby and Aaron. The villainess gave an evil laugh as she walked out of the lab with plans to take over her new dimension.

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