Jack and Jill are in the possession of the magic beans from the Jack and the Beanstalk story. Eventually, Puss, Kitty Softpaws and Humpty Dumpty track them down in their piglet-filled cart and, after battling them, manage to steal to get into the giant's keep.
However, it is revealed later that Jack and Jill were Humpty and Kitty's bosses and intended to take the Golden Goose and trick Puss into being imprisoned in his home town. Later on, the Great Terror arrives to retrieve the Golden Goose, its baby. It is revealed that Jack and Jill were planning to betray Humpty all along, wanting more than just golden eggs but the actual goose. They try to steal the Goose when Humpty had a change of heart and tried to help Puss lure the Great Terror away from San Ricardo, holding their gun to Humpty's head, only for Kitty to fight them off and then be stomped on by the Terror.
During the credits, Jack and Jill are seen in full body casts.
- Jack and Jill are constantly confused to be the secondary antagonists of the first film and one of Humpty's henchmen. This isn't true as Jack and Jill are more malicious and heartless than Humpty as they never intended to stay on Humpty's side from the start and would eventually kill him before taking the goose for themselves.
- Throughout the film, Jack tries convincing Jill that they should have a family, but she assumes they should be taking care of their piglets.