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While Mario is depicted as a hero throughout most of his history, he has been enemies with Donkey Kong and Donkey Kong Jr.. These are his villainous actions.

Coincidentally, Mario in his first game (the original Donkey Kong) was depicted as a naughty carpenter who abused his own pet, Donkey Kong. In the prequel Game & Watch game, Donkey Kong Circus, Mario forces poor Donkey Kong to perform at the circus by juggling pineapples and dodging fireball, and even laughs at him whenever he either gets hit by a fireball or drops a pineapple.

His role just worsened in the sequel, Donkey Kong Jr. as he is actually the villain in that game.

Biography

Donkey Kong Jr. (game)

Wpid-donkey-kong-jr.-screenshot
Mario went on to imprison Donkey Kong in a cage. DK Junior went on to try and rescue his father. Mario attempted to stop the young ape and released various types of traps mainly consisting of animals. Junior eventually did make it to his father and the two returned to Donkey Kong Island just after Donkey Kong made a shot at Stanley the Bugman's greenhouse.

He also whipped the animals that worked for him, eventually known as Mario's slave labor. This makes him extremely evil and cruel, as he also let unnecessarily made them know who was in charge (him) his role was not better before as he abused DK before the events of Donkey Kong. Jr.

Donkey Kong Circus

Mario circus
Mario appears as the antagonist in Donkey Kong Circus. Mario is an audience member who laughs at Donkey Kong, the new attraction of the circus, whenever he drops a pineapple or gets hit by fire. In the end of the game, Mario is shown in the construction site where the first game begins.

Gallery

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           Donkey Kong Villains

Kremlings
King K. Rool | Kaptain Skurvy | Kritter | Klump | Kip | Kass | Kalypso | Kludge | Krusha | Kudgel | Congazuma | Kackle | Klaptrap | Klobber | Koin | Kosha | Kerozene | Kopter | Kuff 'n' Klout | Kutlass | Mega Amp | Dragon Kremling | Kloak
Allies: KAOS | Very Gnawty and Really Gnawty | Master Necky and Master Necky Snr. | Queen B. | Dumb Drum | Flotsam | Clambo | Krow | Kleever | Zinger | Gnawty | Army | King Zing | Manky Kong | Slippa | Belcha | Arich | Squirt | Bleak | Barbos

Tiki Tak Tribe
Tiki Tong | Kalimba | Gong-Oh | Maraca Gang | Wacky Pipes | Cordian | Banjo Bottom | Xylobone | Tiki Tong Tower | Tiki Goon | Screamming Pillar | Tiki Buzz | Tiki Pop | Tiki Bomber | Tiki Tank | Tiki Seeker | Tiki Torch | Tiki Boing
Allies: Mugly | Scurvy Crew | Stu | Mole Train | Mangoruby | Thugly | Colonel Pluck | Awk | Toothberry | Squeeklys | Snaps | Chomps | Snaggles | Squiddicus | Robo-Hand Operator

Snowmads
Lord Fredrik | Pompy | Skowl | Ba-Boom | Fugu | Bashmaster | Tuck | Waldough | Hootz | Fluff | Lemmington
Allies: Dozy | Buffaloafer | Schnautzel | Swooper Dooper | Ice Dragons

Others
Fire | Snapjaw | Mario | Wizpig | Krunch | Crazy Kong | Fire Necky | Sassy Squatch | Davy Bones | B. Locker | Krosshair | Kroctopus | Kong Fu | Cactus/Ghastly King | Hogs | Tusks | Rocs | Dread Kong | Karate Kong | Ninja Kong | Sumo Kong | Ninjape | Iguanagon | Smokey the Dragon | Bluey the Walrus | Bubbler the Octopus | Tricky the Triceratops | Army Dillo | Dogadon | Mad Jack | Puftoss | King Kut Out | Hard Hat | Screech | Polly Roger | Chomps

           TheMario Villains

Koopa Troop
Bowser | Baby Bowser | Count Koopula | Bowser Jr. | Captain Goomba | Chain Chomps | Chauncey | Koopalings: (Larry Koopa | Morton Koopa Jr. | Wendy O. Koopa | Iggy Koopa | Roy Koopa | Lemmy Koopa | Ludwig Von Koopa) | Kamek | Kammy Koopa | King Boo | King Bob-omb | Petey Piranha | Gobblegut | Torkdrift | Goombas | Boos | Piranha Plants | Burt the Bashful | Salvo the Slime | Boom Boom | Pom Pom | Roger the Potted Ghost | Red & Blue Goomba | Goomba King | Koopa Bros | Tutankoopa | Tubba Blubba | General Guy | Lava Piranha | Huff N. Puff | Crystal King |

8 Bit Club
Wart | Mouser | Tryclyde | Birdo | Shy Guys

Secret Society of X-Nauts
Sir Grodus | Lord Crump | Shadow Sirens | Doopliss | Shadow Queen

Shroobs
Elder Princess Shroob | Princess Shroob | Commander Shroob | Sunnycide

Count Bleck's Minions
Count Bleck | Nastasia | O'Chunks | Mimi | Dimentio | Mr. L

Smithy Gang
Smithy | Exor | Mack | Bowyer | Yaridovich | Axem Rangers | Blade | Boomer | Count Down | Cloaker | Domino | Clerk | Manager | Director | Factory Chief

Kremlings
King K. Rool | Kremling Krew | Kritters | Klump | Krusha | Krunch | Klubba

Other
Antasma | Army Dillo | Belome | Big Bad Wolf | Big Bungee Piranha | Big Guy the Stilted | Black Jewel | Bonechill | Booster | Bouldergeist | Brobot | Broodals | Bugaboom | Cackletta | Chuckolator | Cloud N. Candy | Cookatiel | Cortez | Cosmic Mario | Count Bleck's Father | Count Cannoli | Crazy Kong | Croco | Culex | Dark Star | Dino Piranha | Dodo | Donkey Kong | Donkey Kong (Mario VS Donkey Kong) | Draggadon | Eely-Mouth | Fawful | Foreman Spike | Francis | Giga Lakitu | Glamdozer | Gloomtail | Gooper Blooper | Grouchy Possessor | Grubba | Harsh Possessor | Hisstocrat | Hooktail | Hot Roderick | Jojora | Jr. Troopa | Kaptain Skurvy | Kent C. Koopa | King Croacus | King Kaliente | Lena | Madame Broode | Major Burrows | Mario | Mechawiggler | MegaBug | Midbus | Mizzter Blizzard | Mollusque-Lancuer | Nabbit | Necky | Overset Possessor | Peewee Piranha | Peps | Phantamanta | Popple | Portrait Ghosts: (Neville | Chauncey | Bogmire | Biff Atlas | Boolossus | Sir Weston | Vincent Van Gore) | President Koopa | Prince Pikante | Phantom of the Bwahpera | Rabbids | Rabbid Kong | Rawk Hawk | Ricky | Rollodillo | Rudy the Clown | Ruined Dragon | Sabasa | Shadoo | Shake King | Shrewd Possessor | Skellobits | Smorg | Squizzard | Tatanga | Three Little Pigheads | Toadies | Tolstar | Topmaniac | Tough Possessor | Tower Power Pokey | Valentina | Viruses | Waluigi | Wario | Watinga | Whomp King | Wigglers | Wingo | Wracktail | Wizpig | Yellow Belly/Helio

Comics
Bowser (White Knuckle Scorin')

Cartoon
King Koopa | Robot Princess | "Crime Wave" Clyde