While Mario is depicted as a hero throughout most of his history, he has been enemies with Donkey Kong and Donkey Kong Jr. These are his villainous actions.
Coincidentally, Mario in his first game (the original Donkey Kong) was depicted as a naughty carpenter who abused his own pet, Donkey Kong. In the prequel Game & Watch game, Donkey Kong Circus, Mario forces poor Donkey Kong to perform at the circus by juggling pineapples and dodging fireball, and even laughs at him whenever he either gets hit by a fireball or drops a pineapple.
His role just worsened in the sequel, Donkey Kong Jr. as he is actually the villain in that game.
History
Donkey Kong Jr. (game)
He also whipped the animals that worked for him, eventually known as Mario's slave labor. This makes him extremely evil and cruel, as he also let unnecessarily made them know who was in charge (him) his role was not better before as he abused DK before the events of Donkey Kong. Jr.
In the arcade ending, he falls down, gets up and tries to catch Donkey Kong and DK Jr. while swinging his whip, but gets kicked by DK and goes back, runs away. In the NES ending, he falls down and a halo exists over his head. He won't get up and tries to catch them.
Donkey Kong Circus
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