While Mario is depicted as a hero throughout most of his history, he has been enemies with Donkey Kong and Donkey Kong Jr.. These are his villainous actions.
Coincidentally, Mario in his first game (the original Donkey Kong) was depicted as a naughty carpenter who abused his own pet, Donkey Kong. In the prequel Game & Watch game, Donkey Kong Circus, Mario forces poor Donkey Kong to perform at the circus by juggling pineapples and dodging fireball, and even laughs at him whenever he either gets hit by a fireball or drops a pineapple.
His role just worsened in the sequel, Donkey Kong Jr. as he is actually the villain in that game.
History
Donkey Kong Jr. (game)
He also whipped the animals that worked for him, eventually known as Mario's slave labor. This makes him extremely evil and cruel, as he also let unnecessarily made them know who was in charge (him) his role was not better before as he abused DK before the events of Donkey Kong. Jr.
In the arcade ending, after he falls, he tried to catch Donkey Kong and Donkey Kong Jr. with his whip, but got kicked by DK and defeated.
But, in the NES ending, he fell and got defeated, he won't attempt to catch them.
Donkey Kong Circus
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