Mona Lisa is the primary antagonist of the The Sarah Jane Adventures episode "Mona Lisa's Revenge". She is the human form of the painting of the same name.
She was portrayed by Suranne Jones.
On the day Clyde Langer was visiting the art museum after winning a competition, the Mona Lisa painting came to life and came out of her frame. She trapped Phyllis Trupp in the frame in her place, animated a Sontaran blaster, and started looking for the Abomination (her brother). When Sarah Jane confronted her, Mona Lisa trapped her in a painting as well.
Later, Mona Lisa realized she needed to free the Abomination. In order to do that, she needed a Chinese Puzzle Box. She had Clyde draw the puzzle box, and afterwards, she animated it. Unfortunately for her, a drawing of K9 (Sarah Jane's tin dog) that was drawn by Clyde was also animated, and it shot and destroyed the Abomination, freeing all the people who Mona Lisa trapped in paintings and sending her back into her frame, leaving her imprisoned forever.