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Ophiuchus Sam is a descendant of Yosemite Sam. He acts like Yosemite Sam including talking things that Yosemite Sam would say. He attacked a space train carrying the treasure of Freleng. Sometimes, he can't remember what he's saying and has even forgotten his own name. He secretly worked with Zadavia's former General, Deuce to steal Ace's Guardian Strike Sword. He ended up captured, but then released (under the pretense of a brilliant escape) in order to serve as bait for his boss. Much like his ancestor Yosemite Sam, Ophiuchus likes to shoot now and ask questions later. He is very hostile with all of his approaches. Sam is not the brightest, either.

Having to be subtlety helped by the Loonatics to escape (all part of their plan to follow Sam to Deuce).

Sam worked with General Deuce in order to catch the interdimensional train so he could rob it of its riches. Once he was captured and released by the Loonatics he went back to Deuce who knew he was only released so they could find him. Once Deuce finally thinks he has the train, Sam follows. Deuce, not wanting to share the universe with Sam then kicks him off the train.

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Judge Doom | Toon Patrol (Smartass, Greasy, Psycho, Wheezy & Stupid)
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Looney Tunes: Back In Action
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Television
Tiny Toon Adventures
Montana Max | Elmyra Duff | The Brain | Sweetie Bird | Mr. Hitcher | Big Daddy Boo | Johnny Pew | Adults Against Funny Cartoons Chairperson | Duck Vader | Ronald Grump | Tad the Imaginary Friend | Rhoda Queen | Arnold the Pit Bull | Dr. Gene Splicer | Eddy Cougar | Roderick & Rhubella Rat | The Wolverine | Gotcha Grabmore | Amby and Floyd | Boss Tick | Barbette | Foghorn Draghorn | Sappy Stanley | Evil Talking Cake | Silas Wonder | Knuckles Cutlet | Danforth Drake & Margot Mallard | S.N.I.D.E. | Swan Dancers
Animaniacs/Pinky and the Brain
(1993): The Brain | Katie Ka-Boom | Count Dracula | Satan | Walter Wolf | Sid the Squid | Beanie the Bison | Buddy | King Salazar | Fermin Flaxseed | Snowball | Precious | Wally Faust
(2020): Nils Niedhart | Dwayne La Pistol | B.R.A.I.N. | Dr. Walter Grubb | Julia | Nicklewise
Taz-Mania
Tasmanian Devil | Bull and Axle
Duck Dodgers
Duck Dodgers | Martian Commander X-2 | Martian Queen | Martian General Z-9 | Happy Cat (Duck Dodgers)
Loonatics Unleashed
Ringmaster & Otto the Odd | General Deuce | Electro J. Fudd | Optimatus | Massive | Black Velvet | Sylth Vester | Drake Sypher | Weather Vane | Mastermind | Rip Runner | Rupes Oberon | Dr. Dare | Ophiuchus Sam | Pierre Le Pew | Melvin the Martian | Boötes Belida | Adolpho | Stoney and Bugsy | Pinkster Pig
Webtoons
Daffy Duck | Elmer Fudd | Sylvester | Yosemite Sam | Gossamer | Barnyard Dog | Marvin the Martian | Top Salesman | Dr. Zarius | Carrot Monster
The Looney Tunes Show
Daffy Duck | Elmer Fudd | Tasmanian Devil | Sylvester | Yosemite Sam | Marvin the Martian | Wile E. Coyote | Pete Puma | Gossamer | Henery Hawk | Cecil Turtle | Zod Duck | K-9 | Instant Martians | Witch Hazel | Colonel Frankenheimer | Barnyard Dog | Blacque Jacque Shellaque | The Crusher | Shropshire Slasher | Nasty Canasta | Rocky and Mugsy
Wabbit-New Looney Tunes
Daffy Duck | Elmer Fudd | Wile E. Coyote | Yosemite Sam | Carl the Grim Rabbit | Viktor | Claudette Dupri | Hazmats | Foxy Foxworth

Guest Villains
Monstars | Metaluna Mutant | The Man from Planet X | Edward Hyde | Judge Doom | Mr. Chairman | Wicked Witch of the West | Mr. Swackhammer | Daleks

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