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Chemical weapons, human experimentation, SANCTUS was -- is the darkest part of the Agency's history... This place is just evil.
~ Special Agent Iman Avesta on SANCTUS.

SANCTUS is the overarching antagonistic faction of Telltale Games' Batman: The Enemy Within. They are a criminal organization that were once a former black ops science division for The Agency that split from them on the account of the former's viciousness and unethical experiments. From there, they went underground to continue their immoral experiments.

History

Little about the history of SANCTUS is known, but apparently they were once part of the clandestine government organization known as The Agency. Specializing in scientific fields, SANCTUS would conduct black ops experiments for the Agency. They even brought the Gotham City super-criminal known as The Riddler into their ranks following his promotion sometime after the Agency recruited him because of his criminal history and ambitious vision, with Riddler being given a fortune and promised a better future in life. Riddler quickly developed a prestige career in the Agency, which resulted in his promotion to a SANCTUS scientist. Riddler helped SANCTUS by collaborating on their genetic engineering and biochemical weaponization projects, including Project LOTUS. The project involved SANCTUS hoping to turn an enhanced chemical serum known as the LOTUS Virus into something that healed and strengthened the human body to levels of supernatural perfection rather than quickly or slowly ravage it to death.

However, all of the attempts were initially failures which resulted in the deaths of all human test subjects... until Riddler himself was exposed to it, albeit accidentally. However, instead of dying like the other test subjects, Riddler survived, though was driven to permanent insanity, with his body became enhanced to possibly superhuman levels. Experimented on and turned into a guinea pig, Riddler eventually escaped, seething with hatred for SANCTUS but also blaming it's parent organization for it's actions. Thus, Riddler vowed to destroy the Agency while SANCTUS went underground after being cut loose from the Agency when the latter attempted to rectify their mistakes. From there, they became the organization they are as of the events of the games, continuing their work on the LOTUS Virus and trying in vain to repeat their one-time success with Riddler.

Each respectively suffering from an addiction to the super-steroid Venom, a hereditary mental illness that she did not wish to inherit, and a terminally ill wife in need of a cure, the supervillains Bane, Harley Quinn, and Mr. Freeze joined forces with the Riddler, to each get what they wanted; Riddler, his revenge, and the other three, the means by which to make a "miracle cure" using a combination of the LOTUS Virus and Riddler's own blood, while their friend John Doe, a former patient at Arkham Asylum, preferred to get what he wants through different means instead of through the virus. Little did any of them know that even the "success" of Riddler was in fact another failure: the virus, though indeed enhancing his physical abilities, also did irreparable damage to his mind, making him progressively more aberrant, psychotic, and violent.

Ultimately, Bruce Wayne comes to join the Pact undercover at the Agency's request. During the time he spends with the Pact and working with Amanda Waller and the Agency, Bruce learns much of the above (including Catwoman's involvement), before finally getting the rest of the story after the Pact assault SANCTUS' Gotham black-site underneath the seemingly ordinary Bodhi Spa. By this point, between the efforts of the Pact and also the Agency (especially if Bruce earlier broke his cover and thus caused the villains to get there ahead of him and slaughter numerous SANCTUS personnel), the organization appears to be at last disbanded. Waller expresses her intent to salvage some of their technology for her own use, which Bruce/Batman can either support or fight against.

Trivia

  • As a criminal organization involved in human experiments and unethical practices that create multiple supervillains who work for them before breaking away later, they could be likened to Weapon X from Marvel Comics. Both are also opposed by both government organizations (SHIELD and the Agency), and superheroes (Wolverine, the X-Men, and Batman).
  • Likewise, as a criminal organization once part of a greater organization that broke away from them and from there went on to perform many unethical and immoral experiments on humans that created at least one vengeful survivor, they are also somewhat similar to Cerberus from Mass Effect (especially since both are villainous organizations in roleplaying video games).

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Queenie Goldstein | Shere Khan | Tabaqui | Howard Clifford | Ditto | Sebastian | Ann Laurent | King Ghidorah | Rodan | Alan Jonah | Asher Jonah | Emma Russell | Critters | The Banana Splits (Fleegle, Drooper, Snorky & Bingo) | Poppy | Karl | Leo | Cry Baby | Kelly | The Principal | The Biology Teacher | Webby Garton | Steven Dubay | Chris Unwin | Tom Rogan | Arthur Fleck | Penny Fleck | Randall | Clowns (Clown & Ambulance Clown) | Wall Street Three | Penny Fleck's Boyfriend | Rose the Hat | The True Knot | Andrei Sator | Priya Singh | Zelda | Consuella | Esmerelda | Saoirse | Terrance Mendoza | Butch | Mechagodzilla | Apex Cybernetics (Walter Simmons, Ren Serizawa & Maia Simmons) | Shang Tsung | Sub-Zero | Mileena | Reiko | Kano | Kabal | Goro | Reptile | Nitara | Shao Kahn | Al-G Rhythm | Pete | Goon Squad | Baron Vladimir Harkonnen | Count Glossu Rabban | Gabriel | The Analyst | Riddler | Penguin | Carmine Falcone | Joker

Animated Television
The Grinch | Cousin Mel | I.M. Slime | Snow Miser | Heat Miser | General Parvo | Werewolves | North Wind | Hervnick Z. Snerz | Goat | Yes-Man | The Dooka of Yookia | The Dookess of Zookia | Philip Trousers | Marilyn Blouse

Video Games
Sauron | Black Hand of Sauron | Celebrimbor | Tower of Sauron | Hammer of Sauron | Children of Arkham (Vicki Vale, Oswald Cobblepot & Blockbuster) | Catwoman | Two-Face | Falcone Crime Family | Joe Chill | Victor Zsasz | Mr. & Mrs. Vale | Gotham Criminal Triumvirate (Carmine Falcone, Thomas Wayne & Hamilton Hill) | The Pact (Riddler, Harley Quinn, Bane, Mr. Freeze, John Doe & Eli Knable) | The Agency (Amanda Waller & Roger Harrison) | Rumi Mori | Willy Deever | S.A.N.C.T.U.S. | Zog the Eternal | Brûz the Chopper

Shorts
Daffy Duck | Sylvester | Tasmanian Devil | Wile E. Coyote | Elmer Fudd | Yosemite Sam | Marvin the Martian | Instant Martians | Gossamer | Tom | Jerry | Spike | Butch

Music
Crazy Taxi Driver | Cry Baby | Big Bad Wolf | Mr. Potato Head

See Also
Adventure Time Villains | Amblin Entertainment Villains | Aquaman Villains | Archieverse Villains | Arkhamverse Villains | Arrowverse Villains | Batgirl Villains | Batman Villains | Batman Beyond Villains | Batwoman Villains | Ben 10 Villains | Birds of Prey Villains | Black Lightning Villains | Blue Beetle Villains | Blumhouse Productions Villains | Captain Planet Villains | Catwoman Villains | Cats & Dogs Villains | Cartoon Network Villains | Christopher Nolan Villains | Constantine: The Hellblazer Villains | Danmachi Villains | DC Animated Universe Villains | DC Animated Movie Universe Villains | DC Extended Universe Villains | DC Super Hero Girls Villains | DC's Legends of Tomorrow Villains | Doom Patrol Villains | Dune Villains | Eraser Villains | Flash Villains | Game of Thrones Villains | Gotham Villains | Green Arrow Villains | Green Lantern Villains | Gremlins Villains | Hanna-Barbera Cinematic Universe Villains | Harley Quinn Villains | Harry Potter Villains | Injustice Villains | Justice League Villains | Justice League Dark Villains | Justice Society Villains | Laika Villains | Legendary Entertainment Villains | Legion of Super-Heroes Villains | Lethal Weapons Villains | Loonatics Unleashed Villains | Looney Tunes Villains | Lucifer Villains | Mad Max Villains | Melanie Martinez Villains | Middle-Earth Villains | MonsterVerse Villains | Mortal Kombat Villains | New Line Cinema Villains | Pacific Rim Villains | Peacemaker Villains | Pokémon Villains | Powerpuff Girls Villains | Primal Villains | Regular Show Villains | Rick and Morty Villains | Robin Villains | Robot Chicken Villains | Rush Hour Villains | Samurai Jack Villains | Sesame Street Villains | Scooby-Doo Villains | SHAZAM Villains | Sherlock Holmes Villains | Smallville Villains | South Park Villains | Space Jam Villains | Static Shock Villains | Steel Villains | Stephen King Villains | Steven Universe Villains | SWAT Kats Villains | Suicide Squad Villains | Supergirl Villains | Superman Villains | Swamp Thing Villains | Syfy Villains | Teen Titans Villains | Telltale Batman Villains | Terminator Villains | The Banana Splits Movie Villains | The Conjuring Villains | The Hangover Villains | The LEGO Movie Villains | The Matrix Villains | Tim Burton Villains | Tiny Toon Adventures Villains | Tom and Jerry Villains | TMNT Villains | Watchmen Villains | Wild Wild West Villains | Wonder Woman Villains | Xiaolin Showdown Villains | Yu-Gi-Oh! Villains | Young Justice Villains

           Telltale Games.png Villains

Video Games
Toy Mafia (Hugh Bliss) | Brady Culture | Soda Poppers | Jurgen | Strong Bad | The King of Town | Sultry Buttons | Uzi Bazooka | Craig | Trogdor | Queen Bee | Bumblebees | Pudgie-Woo and Tinkie-Wee | Monty Muzzle | The Narrator | General Skun-ka'pe | Anton Papierwaite | Sammun-Mak | "Junior" Max | Charlie Ho-Tep | Billy Yoder | Dr. Laura Sorkin | Miles Chadwick | Walkers | Stranger | Save-Lots Bandits | St. John Family (Andrew, Dan & Brenda) | Jake | Linda | Jolene | Vernon | Crawford Oberson | Logan | Larry | Lilly | Kenny | Marcus Crabtree | Nate | Russell | Roman | Bigby Wolf | Crooked Man | Bloody Mary | Jersey Devil | Georgie Porgie | Vivian | Bluebeard | Ichabod Crane | Grendel | Jack Horner | Woodsman | The Tweedles | Geppetto | Carver's Group (William Carver, Troy & Johnny) | Arvo | Buricko | Vitali | Natasha | Winston | Victor | Ralph | Michelle | Kenny | Jane | Mike | Rhys | Fiona | Handsome Jack AI | August | Hugo P. Vasquez | Vallory | Bossanova | Rudiger | Asher Forrester | Andros | Britt Warrick | Damien | Dezhor zo Raza | Gared Tuttle | Gryff Whitehill | Harys | Ludd Whitehill | Rickard Morgryn | Tazal | Hostile Mobs (Wither Storm) | The Ocelots | Ivor | Soren the Architect | Aiden | Maya | Gill | White Pumpkin | PAMA | Hadrian | Mevia | Em | Norma | Randall | Monroe Colonists (Gabby & Jonas) | Children of Arkham (Vicki Vale, Oswald Cobblepot & Blockbuster) | Catwoman | Two-Face | Falcone Crime Family | Joe Chill | Victor Zsasz | Mr. & Mrs. Vale | Gotham Criminal Triumvirate (Carmine Falcone, Thomas Wayne & Hamilton Hill) | The New Frontier (Joan, Badger & Max) | Scavenger | Romeo | Stella | Prismarine Foes | Warden | The Pact (Riddler, Harley Quinn, Bane, Mr. Freeze, John Doe & Eli Knable) | The Agency (Amanda Waller & Roger Harrison) | Rumi Mori | Willy Deever | S.A.N.C.T.U.S. | The Delta (Lilly, Minerva, Abel, Dorian, Sullene, Gad, Michael, Gina & Armando) | Marlon | Violet

See Also
Game of Thrones Villains | Homestar Runner Villains | Jurassic Park Villains | Minecraft Villains | Telltale Batman Villains | Telltale's The Walking Dead Villains | Wallace and Gromit Villains

           Batman Telltale logo.png Villains

Gotham Criminal Triumvirate
Thomas Wayne | Hamilton Hill | Carmine Falcone

Children of Arkham
Vicki Vale | Oswald Cobblepot | Blockbuster

The Pact
Riddler | Harley Quinn | Bane | Mr. Freeze | John Doe | Eli Knable

The Agency
Amanda Waller | Roger Harrison

Others
Catwoman | Two-Face | Falcone Crime Family | Joe Chill | Victor Zsasz | Mr. & Mrs. Vale | Deadshot | Black Spider | Rumi Mori | Willy Deever | S.A.N.C.T.U.S.

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