She was portrayed by Hannah Gottesman.
Upon hearing that the Guardians and Nebula stole two Annulux batteries after killing the Abilisk, Ayesha intends to kill them all by hiring the Yondu Ravager Clan (led by Yondu Udonta) to track them down. To that end, the Sovereign Chambermaid accompanied Ayesha to the planet Contraxia, helping another maiden in unfolding a blue carpet in front of Ayesha as the latter refuses to walk on the planet's snowy soil.
The Sovereign Chambermaid was later seen receiving a message from Taserface, who gives her the coordinates of Yondu's escape pod heading towards the planet Ego. Before Taserface dies, he demands for the Chambermaid to tell Ayesha of his name that would seal Yondu's fate, but she just laughs upon hearing it, much to his annoyance. Nevertheless, the Chambermaid informed Ayesha of the message, so Ayesha personally sends up a squadron of Omnicrafts to attack the Guardians at Ego's core. Fortunately, Yondu and Nebula utilized the escape pod's lasers to destroy all the Omnicrafts, much to Ayesha's anger.
Following Ego's death, the Chambermaid informs that the Sovereign Council is intending to put Ayesha on trial for wasting their resources on her plot to destroy the Guardians. However, Ayesha calmly informs the Chambermaid that the Council's anger will be subsided as she intends to reveal her latest creation called "Adam Warlock", which she intends to use to kill the Guardians.