While in mythologies such as Sumerian, Ancient Grecian, Ancient Roman, Aboriginal Australian, and many Native American spiders are depicted as either harmless or outright benevolent creatures, other ancient beliefs portrayed them as tricksters and villains.
In Native American Lakota myth, a spider-like trickster named Unktomi was known to trick travelers and heroes out of their clothes.
In ancient Japanese folklore, the legendary Minamoto no Raiko encountered the shapeshifting spider Tsuchigumo, who takes the guise of a young boy to lure the hero into his web, disguised as a house.
In West African myth, Anansi was a trickster deity of creation personified as a spider.
A spider was famously used as the titular main antagonist of the 1829 poem "The Spider and the Fly" by the late Mary Howitt:
"Will you walk into my parlour?" said the Spider to the Fly,
'Tis the prettiest little parlour that ever you did spy;
The way into my parlour is up a winding stair,
And I've a many curious things to shew when you are there."
"Oh no, no," said the little Fly, "to ask me is in vain,
For who goes up your winding stair
-can ne'er come down again."
Spiders likely gained reputations as symbols of evil from the 1842 Biedermeier novella The Black Spider by the late Jeremias Gotthelf, where the spider symbolizes evil works and represents the moral consequences of making a pact with the devil.
Additionally, a spider was famously portrayed as the main antagonist of the children's nursery rhyme "Little Miss Muffet", in which the titular character is frightened by a large spider that causes her to flee.
Spiders' receptions as frightening and predatory creatures have led to them becoming popular antagonists in horror and fantasy, as well as common symbols and icons of Halloween.
Additionally, giant, man-eating spiders are common foes in fantasy, horror, and science fiction, such as with Shelob from The Lord of the Rings franchise, Aragog from the Harry Potter franchise, Kumonga from the Godzilla franchise, and the true form of Pennywise/IT from Stephen King's IT.
In modern folklore, spiders are the subject of many horror-themed urban legends, such as that a human swallows spiders in their sleep, baby spiders can emerge from spider bites, and spiders infesting bananas shipped from overseas. In a South American email hoax that began in 1999 and still spreads to this day, a completely fictitious species of spider known as the South American Blush Spider is reported to live in toilets and nest inside unsuspecting victims' anal cavities.
In cryptozoology, there exist rumors of a Congolese giant spider known as the J'ba Fofi, with reports coming in as early as the 1890s.