The Spy Girls first appeared when kitty, (the leader of them), notices the ugly dolls arrive in perfection. Kitty draws Tuesday & Lydia's attention to them, and does a impression of how Lou would react to seeing the uglydolls.
Nolan comes over and asks the spy girls who Lou is. after kitty shames him for not knowing, the three of them to him to "look, listen, and learn," gesturing up as Lou appears at the top of the stairs.
After Lou's song, the uglydolls start leaving. However, Moxy changers her mind at the last minute and declares that they're staying in perfection and going to the big world.
after sending the uglydolls off with Mandy, Lou tasks the Spy Girls with kidnapping ox in uglyville.
The Spy Girls sneak into Uglyville at night. On the way to kidnap ox, Lydia and Tuesday found out that Kitty stole Lydia's watch and earrings and Tuesday's Lipstick. Though upset, the two of them have to admit, that they look good on kitty.
When they reach to Ox's House, They throw a grappling hook into his window to climb up. instead, the rope gets tangled on Ox and drags him out of the window and into their sack. Ox tries to run away using his ears, but steps on the rake and falls over. The Spy Girls return to Lou's House. where they find out that Ox And Lou have a history together.
Kidnapping Mandy and Moxy
After the Uglydolls are demorized and return to Uglyville, Lou sends the spy girls to Moxy.
Mandy is with her, and before they kidnap her too, they made Evil Smiles. On the way back to Perfection, they meet up with Lou, who tells them that he's changed the plan a little bit. instead of bringing Moxy back to Perfection, he's going to recycle her along with Mandy. He pushes Mandy and Moxy down the recycling chute and leaves with The Spy Girls.
Later after the Gauntlet, Lou reveals he's a prototype, and later betrays them by insultingly calling them "mindless sycophants".
After defeating Lou, The Spy girls now work for Moxy, Due to Lou betraying them. And during the movie's last song, They are in Uglyville, singing how it's cool and unique to be ugly.
- Like Most henchmen, they redeemed themself after getting betrayed by Lou.
- Its unknown why or when they started working for Lou.
- At the end of the movie, Its possible that Lydia is now the leader now.
- When Lou begs for help after being defeated, Lydia answers for them
- When Lou was about to break Mandy's Glasses, you can briefly see Lydia & Kitty making an evil smirk.
- however the spy girls were briefly shown the feel bad about Moxy & Mandy as shown before Lou throws Moxy and Mandy.
- Despite Being Henchwoman of Lou, they did not sing any villain songs before redeeming themselves.
- It Assumed that they now work for Moxy dues to Lou Betraying them after the gauntlet.