The Three Blind Mice are supporting antagonists of the book and film of Dr. No. They are assassins working for Dr. Julius No whose modus operandi is to pretend to be blind beggars in order to lull their victims into a false sense of security. In the film, they were portrayed by Eric Coverly, Charles Edghill and Henry Lopez.
In The Book
The Three Blind Mice are sent to the Queen's Club on the orders of Dr. Julius No in order to assassinate John Strangways, an MI6 agent gathering information on Dr. No. As Strangways leaves the club, he walks past the Three Blind Mice, and throws them a coin just before they gun him down. They then conceal his body in their car and go to his house, where they kill his secretary Mary Trueblood and burn the files he collected on Dr. No.
At the end of the book, Bond runs into them while attempting to escape Dr. No's base. Realising that they killed Strangways, Bond draws his gun and shoots all three of them to death.
In The Film
After R.J. Dent informs Dr. No that Strangways has discovered traces of radioactive material from his base on Crab Key Island, the Mice are sent to kill him. As in the novel, they pretend to be blind beggars and gun down Strangways after he throws them a coin, then go round to his house, where one of them shoots Trueblood and they steal his report.
Several scenes later, the Mice are ordered to kill Bond, however a car arrives before they can do so and they have to leave. Soon after, Miss Taro invites Bond to her house, as part of a trap which allows the Mice to chase him down in a hearse. However, as the Mice fire at Bond, he manages to outsmart them, causing their car to crash and burn with all three still inside.