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The Viruses are anthropomorphic embodiments of disease (albeit in a family-friendly manner) and the main antagonists of the Dr. Mario series as well as the spin-off, Dr. Luigi - like many Mario villains they are comical in appearance and nature but are theoretically very dangerous opponents since they would presumably spread illness across the Nintendo universe if Mario (and later Luigi) didn't keep them at bay.

Due to Dr. Mario being a puzzle-based, simplistic, game the Viruses do not speak nor have much influence but they serve as enemies along the line of famous video-game enemies such as Space Invaders or (fittingly) the original Koopa Troopas, having little backstory but being set as enemies for the hero to defeat.

Much like real-world virsuses the only way to defeat these microscopic creatures was with advanced medicine, symbolised in-game by stacking colored pills ontop of one another to destroy the virus that has the same color as the pills around it.

           TheMario Villains

Koopa Troop
Bowser | Baby Bowser | Count Koopula | Bowser Jr. | Captain Goomba | Chain Chomps | Chauncey | Koopalings: (Larry Koopa | Morton Koopa Jr. | Wendy O. Koopa | Iggy Koopa | Roy Koopa | Lemmy Koopa | Ludwig Von Koopa) | Kamek | Toadies | Kammy Koopa | King Boo | King Bob-omb | Petey Piranha | Gobblegut | Torkdrift | Goombas | Boos | Piranha Plants | Burt the Bashful | Salvo the Slime | Roger the Potted Ghost | Red & Blue Goomba | Goomba King | Koopa Bros | Tutankoopa | Tubba Blubba | General Guy | Lava Piranha | Huff N. Puff | Crystal King |

8 Bit Club
Wart | Mouser | Tryclyde | Birdo | Shy Guys

Secret Society of X-Nauts
Sir Grodus | Lord Crump | Shadow Sirens | Doopliss | Shadow Queen

Shroobs
Elder Princess Shroob | Princess Shroob | Sunnycide

Count Bleck's Minions
Count Bleck | Nastasia | O'Chunks | Mimi | Dimentio | Mr. L

Smithy Gang
Smithy | Exor | Mack | Bowyer | Yaridovich | Axem Rangers | Blade | Boomer | Count Down | Cloaker | Domino | Clerk | Manager | Director | Factory Chief

Kremlings
King K. Rool | Kremling Krew | Kritters | Klump | Krusha | Krunch | Klubba

Other
Antasma | Army Dillo | Belome | Best Fitness Friends | Big Bad Wolf | Big Guy the Stilted | Black Jewel | Bonechill | Booster | Bouldergeist | Brobot | Broodals | Bugaboom | Cackletta | Chuckolator | Cloud N. Candy | Cookatiel | Cortez | Cosmic Mario | Count Bleck's Father | Count Cannoli | Crazy Kong | Croco | Culex | Dark Star | Dino Piranha | Dodo | Donkey Kong | Donkey Kong (Mario VS Donkey Kong) | Draggadon | Eely-Mouth | Fawful | Foreman Spike | Francis | Giga Lakitu | Glamdozer | Gloomtail | Gooper Blooper | Grouchy Possessor | Grubba | Harsh Possessor | Hisstocrat | Hooktail | Hot Roderick | Jojora | Jr. Troopa | Kaptain Skurvy | Kent C. Koopa | King Croacus | King Kaliente | Lena | Madame Broode | Major Burrows | Mario | Mechawiggler | MegaBug | Midbus | Mizzter Blizzard | Mollusque-Lancuer | Nabbit | Necky | Overset Possessor | Peewee Piranha | Peps | Phantamanta | Popple | Portrait Ghosts: (Neville | Chauncey | Bogmire | Biff Atlas | Boolossus | Sir Weston | Vincent Van Gore) | President Koopa | Prince Pikante | Phantom of the Bwahpera | Rabbids | Rabbid Kong | Rawk Hawk | Ricky | Rollodillo | Rudy the Clown | Ruined Dragon | Sabasa | Shadoo | Shake King | Shrewd Possessor | Skellobits | Smorg | Squizzard | Tatanga | Three Little Pigheads | Tolstar | Topmaniac | Tough Possessor | Tower Power Pokey | Valentina | Viruses | Waluigi | Wario | Watinga | Whomp King | Wigglers | Wingo | Wracktail | Wizpig | Yellow Belly/Helio

Comics
Bowser (White Knuckle Scorin')

Cartoon
King Koopa | Robot Princess | "Crime Wave" Clyde

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